| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050172 |
| Chromosome: | chromosome 17 |
| Location: | 5928092 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741950 | BBS1 | Bardet-Biedl syndrome-1 associated protein; (1 of 1) K16746 - Bardet-Biedl syndrome 1 protein (BBS1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGTCCTCACCGATGGCCAGGTGAGCATGTCGTCCCGTTCGCCTATGC |
| Internal bar code: | GCGCTTCTTAGCTTGTAAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1197 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTGTTCTTCGGCGCAAG |
| Suggested primer 2: | TTGCAGTCTAGGCCAAAGGG |