| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050174 |
| Chromosome: | chromosome 3 |
| Location: | 3931127 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171100 | (1 of 77) IPR001623 - DnaJ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTGGAGGCGGCCCAGGAATCTCGCCCTGTGCAAGGCGTGCCGACCCG |
| Internal bar code: | AGCGGTTTTAACTTGGGGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1830 |
| LEAP-Seq percent confirming: | 83.1579 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTCTGGGTATCGAGCCT |
| Suggested primer 2: | GGGAGGCAAGGGAGTTACAC |