Insertion junction: CLIP2.050176_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.050176
Chromosome:chromosome 15
Location:606526
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g636600 FAP391 (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1); Flagellar Associated Leucine Rich Repeat Protein 391 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACGCGAAGGAGGCCCCAGGTGCGTTTCAGGTCGGCCTTCGGTTAGTA
Internal bar code:GTGTGTCTGGGTTCTAAATCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1885
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AATGGAAGAGAACAGCCGCA
Suggested primer 2:CAGAATCGGCGCGAAAGAAG