| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.050176 |
| Chromosome: | chromosome 15 |
| Location: | 606528 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636600 | FAP391 | (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1); Flagellar Associated Leucine Rich Repeat Protein 391 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAGCGACATCCCGTCGGCTGGGGGCCCCGTTGGCCACCCCTCTACGG |
| Internal bar code: | GTGTGTCTGGGTTCTAAATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAATCGGCGCGAAAGAAG |
| Suggested primer 2: | AATGGAAGAGAACAGCCGCA |