| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050184 |
| Chromosome: | chromosome 1 |
| Location: | 650751 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGCAAAACGGCTAGATGCTTATTAACGACTTCCACAGCCGTGGAGT |
| Internal bar code: | CTCTAGCACTGCCTGGATTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1326 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGTAGAGGCGGAGGTCA |
| Suggested primer 2: | GTCCTAGCCAACCCTCAACC |