Insertion junction: CLIP2.050195_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.050195
Chromosome:chromosome 1
Location:5628558
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g040200 WNK2 (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK); WNK protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGGTGACGCTATGCCGCGGCTTCACGGCGCCGCAGTCGGTGCTGGGC
Internal bar code:CAAGCGGTATCGTTCTATTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:5397
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AACAATCAGAGGCGATGGCA
Suggested primer 2:TGCTTTTGACGAAGGCAAGC