| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050208 |
| Chromosome: | chromosome 12 |
| Location: | 3325806 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498700 | CPLD13 | Conserved in the Plant Lineage and Diatoms | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGATCGCATGCATTCCTCCCATGCACCCTGCCGTCGTCCCAGCCATT |
| Internal bar code: | ATGCCTGTTTCAGCGACAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2350 |
| LEAP-Seq percent confirming: | 92.233 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCACAGTGCTCAAAACC |
| Suggested primer 2: | TGGACACCTCTCAAACGCTC |