| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050221 |
| Chromosome: | chromosome 10 |
| Location: | 4959936 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454025 | (1 of 1) PTHR23058//PTHR23058:SF0 - PEROXISOMAL MEMBRANE PROTEIN PEX14 // PEROXISOMAL MEMBRANE PROTEIN PEX14 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGGCGGAGCCGCTGCGAGGTCCACGGATGGGCTGGACAGCCCTGGC |
| Internal bar code: | TCGGCGCGTTTAGGGAAGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTTTCTTGAAGCCCCCT |
| Suggested primer 2: | TGTGTGGTGTCGGAGATGTG |