Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.050224 |
Chromosome: | chromosome 12 |
Location: | 5573511 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g530950 | TGL18 | (1 of 5) PF01734 - Patatin-like phospholipase (Patatin); Putative triacylglycerol lipase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGGGCGCCGCACTCCACGATAGGCTGCTTCAGGACATCCGCACTTGG |
Internal bar code: | GTGACCTAATGTAGGTTTGCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4428 |
LEAP-Seq percent confirming: | 95.2381 |
LEAP-Seq n confirming: | 40 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAAAACTGTTGCCTCTGCC |
Suggested primer 2: | ATCCAGCCACATCCCCTACT |