| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050245 |
| Chromosome: | chromosome 16 |
| Location: | 1783827 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655050 | NON1 | NO-nitrate 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAACATCTGATGCCCCACACTGCTGGTGCAGCCTCACCCGTGGGATG |
| Internal bar code: | GATTCAATCGCAGAGCGAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 26.9231 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGACTACTATCGCGCGG |
| Suggested primer 2: | AAAGAGTCCCAAGTCGGCAG |