Insertion junction: CLIP2.050246_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.050246
Chromosome:chromosome 12
Location:4917006
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g524100 ABH1,CBP80 mRNA cap-binding protein; (1 of 1) K12882 - nuclear cap-binding protein subunit 1 (NCBP1, CBP80) 3'UTR
Cre12.g524150 POLH2,POLI2 (1 of 2) K03509 - DNA polymerase eta (POLH); DNA polymerase eta intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCTCCATCACACCCCGCGCTGCTGTCCTTGCCAATCTCTCACAACCT
Internal bar code:TCACTATATAGAGCATAAGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:169
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATCATGTGGATGAGCGGGTG
Suggested primer 2:GTCGTGAAATGATGACGGCG