| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050256 |
| Chromosome: | chromosome 1 |
| Location: | 786322 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004300 | ASNS,ASN1 | (1 of 1) K01953 - asparagine synthase (glutamine-hydrolysing) (asnB, ASNS); Asparagine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCACGACACTCCACGCCAGTAAAACACGACACAATAGCGCGCAGGCT |
| Internal bar code: | TCGGAATTTCTGCGACAAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2775 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGGCCCATTGTTATGACT |
| Suggested primer 2: | ATGCGTGAATCTGAATGCGC |