Insertion junction: CLIP2.050256_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.050256
Chromosome:chromosome 15
Location:1191913
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g639050 (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGGACTATGACGAGGACGAGGAGGACGAGGACGAGGGCGAGGACGAGG
Internal bar code:CGCTGTACTCTTAATCTATTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2234
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:AACTTACAACACGCACACGC
Suggested primer 2:GACACTCTTCCCGAGCATCC