Insertion junction: CLIP2.050256_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.050256
Chromosome:chromosome 15
Location:1191924
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g639050 (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATAGTCCTCGTCATAGTCCTCGTCGTAGTCTGCCTGCACCGCATCCAGG
Internal bar code:CGCTGTACTCTTAATCTATTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2937
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GACACTCTTCCCGAGCATCC
Suggested primer 2:AACTTACAACACGCACACGC