| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050258 |
| Chromosome: | chromosome 12 |
| Location: | 3517225 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496750 | NUO8 | (1 of 1) K03941 - NADH dehydrogenase (ubiquinone) Fe-S protein 8 (NDUFS8); NADH:ubiquinone oxidoreductase subunit 8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTGACGTTGGGAGCCTGGACACGCCACGCCATACTGGTATGGTTCC |
| Internal bar code: | AGTGAATTACTATCAGTGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1483 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCGTGTTGTCTCCGCCAT |
| Suggested primer 2: | TAGCACACGAAACGCCTTCT |