Insertion junction: CLIP2.050260_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.050260
Chromosome:chromosome 13
Location:2212343
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g578200 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCAACGTGACCGCCGCGGGCCGCCGCCGACAGTACTGACGTGTCCCAG
Internal bar code:CCTATGCCCAGGTGTGAAGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1116
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGCTCCAAGATCGCATCG
Suggested primer 2:CTGCTCCTGCTCTTTCTGCT