Insertion junction: CLIP2.050269_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.050269
Chromosome:chromosome 3
Location:715993
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145887 TAT3,TATC,TATC1 (1 of 1) K03118 - sec-independent protein translocase protein TatC (tatC); TatC-like sec-independent protein translocon subunit intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGGACTGAGGCCCCTGCAAAACACACGCCACGTCGCGAAGCACCTTC
Internal bar code:TTTGCCCCTGCTTCAGTTCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:753
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TACTTGGGGAGGATGGGGAG
Suggested primer 2:CCATTGGAGCCAAGGTACGT