Insertion junction: CLIP2.050273_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.050273
Chromosome:chromosome 16
Location:1086324
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATATCATAATGGCGTGACAGCAGTACGGTACTCACTCCCTCACCTGCTA
Internal bar code:CGTTTATGGGATGGTGAGTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3717
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GTAACTGGTGGGGAGAGTGC
Suggested primer 2:GTCACCCTCCGATCGATCAC