Insertion junction: CLIP2.050286_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.050286
Chromosome:chromosome 17
Location:3801323
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g726983 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACGAGACACTAGCACCAGAGGCTCTGCGCCTCACCCCGGTTTGCCGCC
Internal bar code:GCCCACGAGGCGTGGCGGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1232
LEAP-Seq percent confirming:95.3488
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGAGGTCTGCCAGAACAC
Suggested primer 2:GCGCCAAGTGAGCAATACTG