Insertion junction: CLIP2.050303_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.050303
Chromosome:chromosome 6
Location:4252187
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278252 GOX18,GOX9 (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase; Glyoxal oxidase 18 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTCTCTCTTCCTCTGCTTAGCTTCTCCCGTGTGCGTTGCGAGCAGGTG
Internal bar code:CATGCTTTAAAAAACCCGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1085
LEAP-Seq percent confirming:22.2222
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:TAGAAAATGTCGCCGGCCTT
Suggested primer 2:AATTCGTTGGCTACCTCCCG