| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050304 |
| Chromosome: | chromosome 12 |
| Location: | 4466367 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGTTGCTTGGGGTCACAGTAAGGTGGGATCTGTGTATTAGTGAGCGC |
| Internal bar code: | TATACAGCGTTTCCCATACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3995 |
| LEAP-Seq percent confirming: | 75.7576 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCGTTGGTGGTGTTTG |
| Suggested primer 2: | GCCAACTCAACTCAGGAGCT |