| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050308 |
| Chromosome: | chromosome 1 |
| Location: | 6138240 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043600 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGCCACAGAGGGGTCTTGCGTCGGCCTCTTTAAGACCCAGTGGAAGG |
| Internal bar code: | ACTGACATATGAGGACAAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4821 |
| LEAP-Seq percent confirming: | 95.3846 |
| LEAP-Seq n confirming: | 124 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 130 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTGTTGCCTTGCCTGAC |
| Suggested primer 2: | CTTGGTAGAGGAGGGGAGCT |