| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050308 |
| Chromosome: | chromosome 3 |
| Location: | 6903181 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800370 | (1 of 1) IPR023412 - Ribonuclease A-domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGACGCTTGCGGCCTCAGACTAGAGACGTGATGTGGGCGCAGCCCACTT |
| Internal bar code: | AAACCTTCCTTGTCGGTAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5734 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCTGAGTCCTGAAACCC |
| Suggested primer 2: | CCGCTGGAATGCCGTAATTG |