Insertion junction: CLIP2.050321_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.050321
Chromosome:chromosome 11
Location:1401939
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g801257 (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAATGGGTCTCATCACAACATTGGTAACTACCGCGCCCGCGCACGGCCG
Internal bar code:ACGGAAATTTAGGGAAGATGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:140
LEAP-Seq percent confirming:4.54545
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TAGAACACACGCTGTCCCAC
Suggested primer 2:GCTAGCCAAGCCAGGTACAA