Insertion junction: CLIP2.050328_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.050328
Chromosome:chromosome 6
Location:3949081
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278198 (1 of 1) 1.14.11.33//4.2.99.18 - DNA oxidative demethylase / Alkylated DNA repair protein // DNA-(apurinic or apyrimidinic site) lyase / Phage-T4 UV endonuclease CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTCCGCATCCAAACCCGCGTGTAGGCCTTTGCACACATTCAACAGTTC
Internal bar code:GGTGAAAGAGGGAATCACTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:693
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGACCTAACATCACCACA
Suggested primer 2:AAGCTCATGCCTCATGCCTT