Insertion junction: CLIP2.050331_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.050331
Chromosome:chromosome 1
Location:6848941
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g049132 (1 of 1) PTHR23097//PTHR23097:SF105 - TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCATTGCAGGTCCACACTGTAGATAGAGGCGTGATATCGAACGGTCAGT
Internal bar code:CTCGAGGTTGTACGCAGTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4169
LEAP-Seq percent confirming:90.1961
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCCTACCGCAACACAGT
Suggested primer 2:ACCACCCGTCAATCCAATCC