Insertion junction: CLIP2.050346_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.050346
Chromosome:chromosome 11
Location:1197210
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467686 (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACACCGCGCTCTGCACTCCCTGGGACTCCCGGCATGTCCCCTGCACAC
Internal bar code:TGGGTAGAATCGGCAACATTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1906
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGTACATCTCGAGGTGCTCC
Suggested primer 2:AATGAGGACCCGCTCTACCT