| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050353 |
| Chromosome: | chromosome 1 |
| Location: | 66886 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000400 | AXL3,ERR1 | (1 of 6) PTHR10994:SF61 - RETICULON-LIKE PROTEIN; Arabinose chain extension enzyme like protein 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGAGGCGGGCGCTGGGGTCGGGCCCCAGCACCGCCTTGGGCATGGCG |
| Internal bar code: | TCTCGCAGGTTTGCTGAACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3351 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTTCATGCGGCAATGGC |
| Suggested primer 2: | CGCTACCAGCTACCTGTACG |