| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050380 |
| Chromosome: | chromosome 2 |
| Location: | 6649006 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389950 | OAT1 | O-acetyltransferase-related protein; (1 of 1) 2.3.1.45 - N-acetylneuraminate 7-O(or 9-O)-acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGATCGCGGCTTGCTTCCTGTTCATCTTCGTGACGTGGGACCTCAA |
| Internal bar code: | AACCGTGGAGGTTCGAGCTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2403 |
| LEAP-Seq percent confirming: | 88.5714 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGCAGGCAGTAGATGTG |
| Suggested primer 2: | GTCGGGAGCATATGGGTCTG |