| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050385 |
| Chromosome: | chromosome 9 |
| Location: | 6532002 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415850 | CAG3 | (1 of 1) PTHR13061:SF11 - GAMMA CARBONIC ANHYDRASE 1, MITOCHONDRIAL-RELATED; Gamma carbonic anhydrase 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAATGAGTGGGCTCAAGCGTCAGGAGGACGTTTTGGCAGGCGACGCC |
| Internal bar code: | CTAGGTTCAGCAGGGTGTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 344 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACTGCAAAGACCCAAAG |
| Suggested primer 2: | GGGGCTTTCTACGTGACACA |