| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.050415 |
| Chromosome: | chromosome 12 |
| Location: | 8952999 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545500 | (1 of 140) IPR002048 - EF-hand domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCCCTCTTCGCCTGCTCACCCTTCCTGCGCGCCTCACCCGACGCCAC |
| Internal bar code: | GTGTTTTAATGTGTACGCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3513 |
| LEAP-Seq percent confirming: | 76.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGTGCAGGTGGTTATG |
| Suggested primer 2: | GTTTGACGACAGGGAGGAGG |