| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.050416 |
| Chromosome: | chromosome 3 |
| Location: | 5226698 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACATGGGCATGCCCTCACCCTGCCTTGCCTAACAGGCCCTGCCCGTTT |
| Internal bar code: | TAGACGGTCGTCCTCCTACCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1301 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTTGCATGTCTCATGGT |
| Suggested primer 2: | CCTCCAAACCCACACCAACT |