| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050424 |
| Chromosome: | chromosome 14 |
| Location: | 2061162 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621351 | (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCGCCCAATCCAGAAGGGGAGGAGCGGTGGCTTGCTTCCACCATTGCC |
| Internal bar code: | CCCAGCCGAGGTTTTGAGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTTTCGGTTGCGGTTGT |
| Suggested primer 2: | GAAGGAGAGGAGGGGAGGAG |