| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050429 |
| Chromosome: | chromosome 11 |
| Location: | 649346 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467613 | (1 of 1) IPR000742//IPR001881 - EGF-like domain // EGF-like calcium-binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGAGAACCTGCCGCCTTTCCATGCCTACATCCAACTCACCCGAGAAA |
| Internal bar code: | CGTTCGAGGCTGTAATGCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1621 |
| LEAP-Seq percent confirming: | 11.7647 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCTGGAATGGTGGCTTG |
| Suggested primer 2: | AGTCGAGGGTGCAATTGGAG |