| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.050437 |
| Chromosome: | chromosome 3 |
| Location: | 5631410 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186050 | (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCAAAACATGGAGAAAGAGCGGACTTCTGGAGGCAGCAACCGCAGCCA |
| Internal bar code: | ATGGTTTTCCCGGACAGTGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1710 |
| LEAP-Seq percent confirming: | 89.2857 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTTTGGAACTGCTCGCG |
| Suggested primer 2: | GGGTAGTAGCGTGGATTCGG |