Insertion junction: CLIP2.050452_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.050452
Chromosome:chromosome 10
Location:5934699
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre10.g461000 (1 of 1) K14772 - U3 small nucleolar RNA-associated protein 20 (UTP20) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCATGCCTTCGCACACATCCCTCGCACACCCGTGCTGCTCACCCAACG
Internal bar code:CTTGGAGCAGGTGTAGCGGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:849
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGTAGCAGGCCGTAGTAG
Suggested primer 2:TTCCCGTGGGTTAGTTCGTG