Insertion junction: CLIP2.050471_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.050471
Chromosome:chromosome 17
Location:3470018
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g723800 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAACCACGTCACTGACCAACCCAGGGCAGGGTGCCCACCCTAATCCAG
Internal bar code:GCAACCCCGCTGTGACTTCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3274
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCAAGCTTGGGTTTGCA
Suggested primer 2:TTACTTCACCACGGCTGACC