| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050485 |
| Chromosome: | chromosome 3 |
| Location: | 8032010 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201500 | (1 of 1) PF16740 - Spindle and kinetochore-associated protein 2 (SKA2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACGTCGCGACCCGTGCCAATGCGGCATTGAGGTCTTGCCGTGACATCT |
| Internal bar code: | TTGGTCGGCTAGACAAACGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3134 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGATCAAGTTGAGCATGCG |
| Suggested primer 2: | AGAAGTATCTGCCGTACGCG |