Insertion junction: CLIP2.050491_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.050491
Chromosome:chromosome 16
Location:2536300
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g660750 MKS6,CC2D2A (1 of 1) K19352 - coiled-coil and C2 domain-containing protein 2A (CC2D2A); Coiled-coil and C2 domain containing 2A CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTCCAGCTCCGCCGTCACCAGCAGCACGTCCAGCCGCTCACGGGTCA
Internal bar code:ATATTTAGTGTTTGAATAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:210
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTCAAGGAGGAGGAGGAG
Suggested primer 2:CACACACACACACACACACG