| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050504 |
| Chromosome: | chromosome 9 |
| Location: | 5301887 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407350 | NAT23 | N-acetyltransferase; (1 of 4) IPR006917//IPR011256 - SOUL haem-binding protein // Regulatory factor, effector binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAACATTGTGAGAAAGCGGCGCCGCAGGGGTGCGGGGAGCCGGACGT |
| Internal bar code: | TTCGGTCTCATGACCGTAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 125 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAGCCTCAAGAGCAGAG |
| Suggested primer 2: | GGAAGGGCAGGAAGAAGGAC |