| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.050505 |
| Chromosome: | chromosome 10 |
| Location: | 5139985 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455451 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCAGGACTGAACCTGGCCAAGGTGGGGGAGACTGGGGTGGGGGAGAAG |
| Internal bar code: | GTTTTCACGTTGGGCATGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 53.8462 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACGAGGGGGTTAGTTTG |
| Suggested primer 2: | ACCCGATGCAATAGAGCGAG |