Insertion junction: CLIP2.050506_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.050506
Chromosome:chromosome 1
Location:717110
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g003950 (1 of 28) PTHR31600:SF2 - TINY MACROCYSTS PROTEIN B-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTGATCGGCCACACTACCGGGAACTTCTGCTCCTTGAAGCACCAGGCC
Internal bar code:TGTTAAAAATGCATAGAAGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1934
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCGGCCAAGTGCAATTTG
Suggested primer 2:GTGAGGGACAAGTGAGGTGG