Insertion junction: CLIP2.050507_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.050507
Chromosome:chromosome 1
Location:3703713
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g024100 (1 of 19) IPR018392 - LysM domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACACGTTGGCGCCACGCACCAGCAAGCTGCGCTCGCGTGGCGCGGGC
Internal bar code:AGTGTATTAGTAACCACACTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:815
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACTACTGCGACCACCAC
Suggested primer 2:GATTCGCTGGCATCGTTGTC