| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.050508 |
| Chromosome: | chromosome 6 |
| Location: | 3327648 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277650 | BES4 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAGCGGAACAGCGTGATGCAGTGCTCTAGGTAGCGCTTGCGGGAGCG |
| Internal bar code: | CGTATTGAGCCGCGGTCGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTCCTTGATTGCAGCC |
| Suggested primer 2: | ACACAAGCATCGCTTCCTCA |