| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.050516 |
| Chromosome: | chromosome 10 |
| Location: | 1506590 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428678 | GID1,MNMG1,MNMG | (1 of 1) K03495 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme (gidA, mnmG, MTO1); tRNA uridine 5-carboxymethylaminomethyl modification enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACCCCTCCCTTCCCGGCACCTGCCCTCCTGCGCCTCCACCGGCTCTG |
| Internal bar code: | GTTTTGGTTAGAAGAATCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 24.1379 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTGCTGCTTATGGTTGT |
| Suggested primer 2: | CACACAGCCTCACAAACACG |