| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050524 |
| Chromosome: | chromosome 7 |
| Location: | 2594993 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330050 | (1 of 1) IPR000571//IPR029063 - Zinc finger, CCCH-type // S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAGAGGCCTGCCAATTCACGCACCCTCCAGCCCGCGGGGGCGCTTCC |
| Internal bar code: | GTTGCTGGCATGTCAACGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 739 |
| LEAP-Seq percent confirming: | 9.67742 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCCCCTCTCCCTGAAGA |
| Suggested primer 2: | GTTTGTAGGGCAAGTGCAGC |