Insertion junction: CLIP2.050527_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.050527
Chromosome:chromosome 5
Location:41428
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g241650 (1 of 1) K01303 - acylaminoacyl-peptidase [EC:3.4.19.1] (APEH) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACACGCCGCGAGGTTGTCAAGGTCAGCCAGGGCGCGGTGGGGGATGG
Internal bar code:CTTACCCGCGAAATAGGACCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1255
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAACACCACACCCAACTTGC
Suggested primer 2:TGAAGCGCTGAACAAACGTG