| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.050530 |
| Chromosome: | chromosome 16 |
| Location: | 1195847 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650625 | (1 of 1) PTHR11757:SF3 - OLIGOPEPTIDASE B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCTGAGGCTGGTGAGACCTAAGGCGAAGGCAGAACAGCAGGCGCTTC |
| Internal bar code: | GTAATGCACATCTAAGACCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 53.8462 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGCAAACATCGTACTGGA |
| Suggested primer 2: | CTACTGCGATGTTGGTTGCG |