| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050535 |
| Chromosome: | chromosome 16 |
| Location: | 3170130 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665364 | GAK,GDAK1 | (1 of 1) K17530 - doublecortin-like kinase 3 [EC:2.7.11.1] (DCLK3); Gliding Associated Kinase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGGTTGATTGGCTTGCGGGGTCGTTGGACGTTTGTGCATGGTACCT |
| Internal bar code: | ACAATGGGCACTGAGTGGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2852 |
| LEAP-Seq percent confirming: | 59.2593 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCCAGTAGACGTCCAAA |
| Suggested primer 2: | TTAACCAGTAGGCCCAAGCG |