Insertion junction: CLIP2.050561_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.050561
Chromosome:chromosome 9
Location:1576759
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399000 SDR18 Short-chain dehydrogenase/reductase; (1 of 5) IPR002198//IPR002347//IPR003560//IPR013968 - Short-chain dehydrogenase/reductase SDR // Glucose/ribitol dehydrogenase // 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase // Polyketide synthase, ketoreductase domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACAACATCAACAACCCTAACACCCCCCCAATATCACATGCCCG
Internal bar code:GTCAGACTCGCATGGCAAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:870
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCGGGATGACTACGTGG
Suggested primer 2:GGTGGGTAGTGGTGGTGATG